SPECIALIZING IN NEURONAL CiRCUITS AND BEHAVIOR
YOUR SOURCE FOR:
-superior retrograde vectors - nontoxic and persistent
-local vectors for behavioral studies
-cell type-specific vectors
EXTENSIVE INVENTORY OF IN-STOCK VECTORS, INCLUDING:
-cre and flpo
CRE-DEPENDENT VERSIONS OF ALL VECTORS ARE IN STOCK
The viral gene transfer core was established in 2008 by the Picower Institute and the McGovern Institute to make viral vectors accessible to the MIT neuroscience community. Directed by Rachael Neve, its overall goals are to provide the most advanced viral vector technologies, and to drive the development of new applications that will benefit neuroscience research.
Viral vectors are a key technology for neuroscience research, allowing delivery of genes into the brain to manipulate brain function. In addition to their speed and cost advantages, viruses can be delivered with spatial and temporal specificity, and in some cases can be engineered to produce cell-type specificity of expression. Importantly, viruses can also be used for genetic manipulation of species for which germ-line transgenic methods are not feasible.
The production of viruses requires special expertise, and given the range of vectors now available from the core, it does not make sense for individual labs to spend time and resources to develop the necessary expertise to make these vectors. The core currently offers many different types of vectors, all of which work effectively in behavioral paradigms. Included in the service are packaging, vector construction and advisory consultations. The core also offers AAV packaging through an external provider, Virovek, with whom the core has negotiated a discount. These AAV vectors, the best in the world, are guaranteed to have a minimum titer of 1 x 113 vg/ml.
The core has an active research and development program, resulting in the continual addition of new types of vectors.
The core operates as a fee-for-service facility, in accordance with MIT policies for core facilities. Its primary purpose is to serve the MIT neuroscience community, but some services are also available to external academic or corporate users. Please refer to the Services and Ordering page for details.
The core occupies a 530-sq-ft laboratory on the 6th floor of the Brain and Cognitive Sciences Complex. For more information please contact the director, Rachael Neve.
Image: Retrogradely transported HSV expressing cre and mCherry intersects with AAV expressing cre-dependent CHR2-EYFP. Edward Nieh (Kay Tye lab)